Poster Title (Current Submission)

Antibody Mediated Identification of Cyclin G2 and its Role in Cell Cycle Arrest

Presenter Information

Eric Mou, University of IowaFollow

Major(s)

Biomedical Engineering

Mentor Department

Pharmacology

Presentation Date

December 2009

Abstract

Cancer cells have lost control of their divisional regulation, leading to uncontrolled growth. This may eventually lead to invasion (intrusion on and destruction of nearby tissues) and metastasis (spread to other locations in the body via the lymphatic system or bloodstream). In the cell, increased levels of the cell cycle protein Cyclin G2 have been correlated with cell cycle arrest. It has been shown that in many cancer cells, the level of G2 is significantly decreased. Thus, methods of detecting Cyclin G2 levels are important in determining its exact role in cancer development. To accomplish this, antibodies specific to Cyclin G2 were generated by animal immunization, sequentially purified, and then applied to cell samples through immunoblotting. The extent of this antibody attachment allowed for the accurate quantification and evaluation of Cyclin G2 levels in cells.

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Dec 3rd, 12:00 AM

Antibody Mediated Identification of Cyclin G2 and its Role in Cell Cycle Arrest

Cancer cells have lost control of their divisional regulation, leading to uncontrolled growth. This may eventually lead to invasion (intrusion on and destruction of nearby tissues) and metastasis (spread to other locations in the body via the lymphatic system or bloodstream). In the cell, increased levels of the cell cycle protein Cyclin G2 have been correlated with cell cycle arrest. It has been shown that in many cancer cells, the level of G2 is significantly decreased. Thus, methods of detecting Cyclin G2 levels are important in determining its exact role in cancer development. To accomplish this, antibodies specific to Cyclin G2 were generated by animal immunization, sequentially purified, and then applied to cell samples through immunoblotting. The extent of this antibody attachment allowed for the accurate quantification and evaluation of Cyclin G2 levels in cells.