Poster Title (Current Submission)
Major(s)
Biochemistry
Minor(s)
Art, Chemistry
Other Mentor Department
Internal Medicine
Abstract
Blood brain barrier(BBB) serves an important protective role for the brain, it also blocks access of most therapeutic reagents and is thus an obstacle for the treatment of many disorders of the nervous system. To determine whether intravenously administered experimental therapeutic reagents are able to cross the BBB in animal models of disease, it is necessary to determine the location of the reagents relative to the vasculature; methods for labeling the vasculature are useful for such studies. Here, we carried out immunofluorescent labeling of the vasculature in mouse brain tissue sections with an antibody specific for von Willebrand Factor, a protein expressed on the surface of brain endothelial cells.
Anti-vWF Immunofluorescence in Mouse Brain
Blood brain barrier(BBB) serves an important protective role for the brain, it also blocks access of most therapeutic reagents and is thus an obstacle for the treatment of many disorders of the nervous system. To determine whether intravenously administered experimental therapeutic reagents are able to cross the BBB in animal models of disease, it is necessary to determine the location of the reagents relative to the vasculature; methods for labeling the vasculature are useful for such studies. Here, we carried out immunofluorescent labeling of the vasculature in mouse brain tissue sections with an antibody specific for von Willebrand Factor, a protein expressed on the surface of brain endothelial cells.