Poster Title (Current Submission)

Direct Evidence Of Neurofibromatosis II Protein – Merlin – Inhibiting Glial Growth Through The ErbB2 Receptor

Major(s)

Integrative Physiology

Minor(s)

Spanish

Mentor Name

Dr. Marlan Hansen

Other Mentor Department

UI Department of Otolaryngology

Presentation Date

March 2010

Abstract

Merlin (Moesin-Ezrin-Radixin-Like) is a tumor suppressor protein predominantly found in nervous tissue. It is a cytoplasmic protein that links actin filaments to the cytoskeleton and helps regulate cell signaling.

It is unknown how exactly Merlin suppresses tumor growth. Nonetheless, mutated, inactive Merlin can commonly be found in neoplasms, including vestibular schwannomas (see Figure 1).

Studies suggest that Merlin correlates with cell cycle entry via interactions with certain transmembrane receptors, like Human Epidermal Growth Factor Receptor 2 (ErbB2).

To provide direct evidence of Merlin interacting with ErbB2, a DNA construct with Merlin tagged with CFP (cyan florescent protein) will be biologically engineered. Cells lacking functional Merlin will be transfected with this construct. Then using fluorescent resonance energy transfer (FRET) microscopy, Merlin’s molecular interactions with ErbB2 will be monitored.

SURF 2010 Prospectus.doc (120 kB)
2010 SURF research prospectus

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SURF 2010 Prospectus.doc (120 kB)
2010 SURF research prospectus

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Mar 27th, 12:00 AM

Direct Evidence Of Neurofibromatosis II Protein – Merlin – Inhibiting Glial Growth Through The ErbB2 Receptor

Merlin (Moesin-Ezrin-Radixin-Like) is a tumor suppressor protein predominantly found in nervous tissue. It is a cytoplasmic protein that links actin filaments to the cytoskeleton and helps regulate cell signaling.

It is unknown how exactly Merlin suppresses tumor growth. Nonetheless, mutated, inactive Merlin can commonly be found in neoplasms, including vestibular schwannomas (see Figure 1).

Studies suggest that Merlin correlates with cell cycle entry via interactions with certain transmembrane receptors, like Human Epidermal Growth Factor Receptor 2 (ErbB2).

To provide direct evidence of Merlin interacting with ErbB2, a DNA construct with Merlin tagged with CFP (cyan florescent protein) will be biologically engineered. Cells lacking functional Merlin will be transfected with this construct. Then using fluorescent resonance energy transfer (FRET) microscopy, Merlin’s molecular interactions with ErbB2 will be monitored.