Poster Title (Current Submission)
Neural Differentiation of Human Skin Keratinocytes
Major(s)
Exercise Science (Integrative Physiology)
Minor(s)
Chemistry
Mentor Name
Jackie Bickenbach
Other Mentor Department
Anatomy and Cell Biology
Abstract
Embryonic transcription factor OCT4, is critical for maintaining cells in the undifferentiated state. We demonstrate that by transient transfection with OCT4, adult human skin keratinocytes can be reprogrammed to be more embryonic stem cell-like i.e. pluripotent. Additionally, we will illustrate through RT-PCR, Western Blot, and images of morphological changes in cell structure that these cells can be differentiated into neuronal cells with exposure to neurotrophic factors.
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Neural Differentiation of Human Skin Keratinocytes
Embryonic transcription factor OCT4, is critical for maintaining cells in the undifferentiated state. We demonstrate that by transient transfection with OCT4, adult human skin keratinocytes can be reprogrammed to be more embryonic stem cell-like i.e. pluripotent. Additionally, we will illustrate through RT-PCR, Western Blot, and images of morphological changes in cell structure that these cells can be differentiated into neuronal cells with exposure to neurotrophic factors.