Title

[Detection of PTCH gene mutations in odontogenic keratocysts by SSCP and DNA sequencing]

Document Type

Speech/Presentation/Lecture

Peer Reviewed

1

Publication Date

8-24-2006

Journal/Book/Conference Title

Hua Xi Kou Qiang Yi Xue Za Zhi

Abstract

OBJECTIVE:

To investigate PTCH gene mutations in odontogenic keratocysts (OKC).

METHODS:

PCR-SSCP and DNA sequencing were used to analyze the PTCH gene mutations in 12 OKCs, including 10 sporadic and 2 nevoid basal cell carcinoma syndrome (NBCCS) associated OKC.

RESULTS:

Four mutations were identified in 4 cysts, among which two germline mutations were associated with NBCCS and 2 somatic mutations were in 2 unrelated sporadic cases. In addition, eight previously reported polymorphisms in the PTCH gene were also found in 10 cases.

CONCLUSION:

The present study indicated that both sporadic and NBCCS-related OKCs could carry PTCH gene mutation. Thus, mutational inactivation of PTCH gene may play a significant role in the pathogenesis of OKC.

This document is currently not available here.

Share

COinS
 

URL

http://ir.uiowa.edu/lib_pubs/109