Iowa Research Online

Title

Efficacy of polymeric encapsulated C5a peptidase-based group B streptococcus vaccines in a murine model.

Authors

Donna A. Santillan, University of Iowa, Iowa City, IAFollow
Karishma K Rai
Mark Santillan, University of IowaFollow
Yogita Krishnamachari
Aliasger K Salem
Stephen K. Hunter, University of IowaFollow

Document Type

Article

Peer Reviewed

1

Publication Date

9-1-2011

Journal/Book/Conference Title

American journal of obstetrics and gynecology

Volume

205

Abstract

OBJECTIVE: The purpose was to examine in mice the efficacy of various polymeric-encapsulated C5a peptidase vaccine formulations in eliciting a long-term immune response and preventing group B streptococcus (GBS) infection.

STUDY DESIGN: C5a peptidase was encapsulated in semipermeable microspheres of poly(lactide-coglycolide) (PLGA). Female ICR mice were immunized with 0, 10, or 30 μg of encapsulated C5a peptidase within 2 different formulations of PLGA polymers. Booster doses were given at weeks 4 and 8. Antibody responses were measured by enzyme-linked immunosorbent assay at weeks 4, 8, 11, and 40. Vaginal challenges with GBS types 1a, III, and V were performed at week 12.

RESULTS: Thirty microgram doses of the 75:25 and 50:50 PLGA formulations generate the highest and most sustained C5a peptidase-specific immune responses. Mice that received encapsulated C5a peptidase were significantly protected from vaginal colonization compared with mice that received empty microspheres.

CONCLUSION: Encapsulated C5a peptidase elicited significant immune responses and protection against a GBS challenge. C5a peptidase microsphere encapsulation has potential as a GBS vaccine.

Keywords

Adhesins, Bacterial, Animals, Endopeptidases, Female, Mice, Mice, Inbred ICR, Microspheres, Streptococcal Infections, Streptococcal Vaccines, Streptococcus agalactiae