Title

Morphologic and immunologic characterization of human basophils developed in cultures of cord blood mononuclear cells

Document Type

Article

Peer Reviewed

1

Publication Date

1-1-1985

NLM Title Abbreviation

J Immunol

Journal/Book/Conference Title

Journal of immunology (Baltimore, Md.: 1950)

PubMed ID

2578052

Abstract

Selective growth of human basophilic granulocytes was obtained in suspension cultures of mononuclear cells from umbilical cord blood. Approximately 50 to 80% of nonadherent cells recovered from 2- to 3-wk-old cultures contained metachromatic granules, and these cells were identified as human basophilic granulocytes by electron microscopy. Histamine content of cultured human basophils was comparable to that in peripheral blood basophils. Cultured basophils bear 2.7 to 3.7 X 10(5) IgE receptors per cell that bind both human IgE and rodent IgE with comparable affinity. Average equilibrium constants of the receptors for human IgE and mouse IgE were 2.56 +/- 0.88 X 10(9) M-1 and 1.85 +/- 0.86 X 10(9) M-1, respectively. The cell-surface component of the IgE receptors on cultured basophils has a m.w. of 64,000. Cultured basophils could be passively sensitized with human IgE and mouse IgE monoclonal antibody, and sensitized basophils released characteristic cytoplasmic granules and both histamine and arachidonate upon challenge with either anti-human IgE or antigen. Incubation of cultured basophils with ionophore A23187 or F-Met-Leu-Phe resulted in histamine release. However, compound 48/80 failed to induce histamine release from the cells.

Keywords

Antigens, Surface/analysis, Arachidonic Acid, Arachidonic Acids/metabolism, Basophils/cytology/immunology/ultrastructure, Carbon Radioisotopes/analysis, Cells, Cultured, Female, Fetal Blood/cytology, Fluorescent Antibody Technique, Granulocytes/cytology, Histamine Release, Humans, Immunization, Passive, Immunoglobulin E, Microscopy, Electron, Monocytes/cytology, Pregnancy, Receptors, IgE, Receptors, Immunologic/analysis

Published Article/Book Citation

Journal of immunology (Baltimore, Md.: 1950), 134:1 (1985) pp.532-540.

This document is currently not available here.

Share

COinS
 

URL

http://ir.uiowa.edu/obgyn_pubs/568