Title

Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin

Document Type

Article

Peer Reviewed

1

Publication Date

12-1-2003

Journal/Book/Conference Title

American Journal of Physiology: Lung Cellular and Molecular Physiology

DOI of Published Version

10.1152/ajplung.00064.2003

Abstract

Chronically inhaled endotoxin, which is ubiquitous in many occupational and domestic environments, can adversely affect the respiratory system resulting in an inflammatory response and decreased lung function. Surfactant-associated protein A (SP-A) is part of the lung innate immune system and may attenuate the inflammatory response in various types of lung injury. Using a murine model to mimic occupational exposures to endotoxin, we hypothesized that SP-A gene expression and protein would be elevated in response to repeat exposure to inhaled grain dust and to purified lipopolysaccharide (LPS). Our results demonstrate that repeat exposure to inhaled endotoxin, either in the form of grain dust or purified LPS, results in increased whole lung SP-A gene expression and type II alveolar epithelial cell hyperplasia, whereas SP-A protein levels in lung lavage fluid are decreased. Furthermore, these alterations in SP-A gene activity and protein metabolism are dependent on an intact endotoxin signaling system.

Keywords

Sustainability

Published Article/Book Citation

The definitive version was published in American Journal of Physiology: Lung Cellular and Molecular Physiology, 285: (2003) pp.L1337-L1344. DOI:10.1152/ajplung.00064.2003.

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URL

http://ir.uiowa.edu/oeh_pubs/58