Document Type

Article

Peer Reviewed

1

Publication Date

1-1-2014

NLM Title Abbreviation

Redox Biol

Journal/Book/Conference Title

Redox Biology

PubMed ID

25009786

DOI of Published Version

10.1016/j.redox.2014.06.006

Abstract

Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved in these effects.

Keywords

OAfund, Stem cells, Viral carcinogenesis, Mammary cancer, Oxidative stress, Antioxidant enzymes, Aldehyde dehydrogenase positive

Journal Article Version

Version of Record

Published Article/Book Citation

Redox Biology 2: (2014) pp. 847-854. doi:10.1016/j.redox.2014.06.006

Rights

Copyright © 2014 The Authors. Published by Elsevier B.V.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

Included in

Radiology Commons

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URL

https://ir.uiowa.edu/radiationoncology_pubs/3