Document Type


Peer Reviewed


Publication Date


NLM Title Abbreviation

Int J Endocrinol

Journal/Book/Conference Title

International Journal of Endocrinology

PubMed ID


DOI of Published Version


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Total Pages



Deficiency of sex hormones and excessive alcohol consumption are factors that have been related to alterations in the pattern of bone mineralization and osteoporosis.The aim of this study was to evaluate possible alterations in the calcium/phosphorus (Ca/P) ratio in the femur of rats subjected to sex hormone deficiency and/or alcohol consumption. Methods. Female andmaleWistar rats (𝑛 = 108) were divided into ovariectomized (Ovx), orchiectomized (Orx), or sham-operated groups and subdivided according to diet: alcoholic diet (20% alcohol solution), isocaloric diet, and ad libitum diet. The diets were administered for 8 weeks. The Ca/P ratio in the femur was analyzed by energy dispersive micro-X-ray spectrometer (𝜇EDX). Results. Consumption of alcohol reduced the Ca/P ratio in both females and males. The isocaloric diet reduced the Ca/P ratio in females. In groups with the ad libitum diet, the deficiency of sex hormones did not change the Ca/P ratio in females or males. However, the combination of sex hormone deficiency and alcoholic diet presented the lowest values for the Ca/P ratio in both females and males. Conclusions. There was a reduced Ca/P ratio in the femur of rats that consumed alcohol, which was exacerbated when combined with a deficiency of sex hormones.




The authors acknowledge financial support from National Council for Scientific and Technological Development (CNPq-Brazil). The authors would also like to thank Ms. Anna Okulist for proofreading this paper. Karina Bortolin Lodi received a scholarship from the Brazilian governmental research agency, National Council for Scientific and Technological Development (CNPq-Brazil), to do this work.

Journal Article Version

Version of Record

Published Article/Book Citation

International Journal of Endocrinology Volume 2016 (2016), Article ID 3797139, 9 pages


Copyright © 2016 Karina Bortolin Lodi et al.

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This work is licensed under a Creative Commons Attribution 4.0 License.