Document Type

Article

Peer Reviewed

1

Publication Date

12-30-2015

NLM Title Abbreviation

J Adv Prosthodont

Journal/Book/Conference Title

Journal of Advanced Prosthodontics

PubMed ID

26816577

DOI of Published Version

10.4047/jap.2015.7.6.468

Abstract

PURPOSE: The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time.

MATERIALS AND METHODS: This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity).

RESULTS: A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2.

CONCLUSION: The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.

Keywords

16S rDNA gene, Denture plaque, Framework, Microorganism, Removable partial denture

Granting or Sponsoring Agency

The State of Sao Paulo Research Foundation, Brazil

Grant Number

01058-3

Journal Article Version

Version of Record

Published Article/Book Citation

J Adv Prosthodont. 2015 Dec;7(6):468-474. http://dx.doi.org/10.4047/jap.2015.7.6.468

Rights

Copyright © 2015 The Korean Academy of Prosthodontics. Posted by permission.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

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URL

https://ir.uiowa.edu/dentistry-preventive_pubs/2