NLM Title Abbreviation
Braz J Oral Sci.
Brazilian Journal of Oral Sciences
DOI of Published Version
Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue.
To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats.
Fifty-four female rats were first divided equally into two groups: ovariectomized Ovx and simulated ovariectomy Sham. One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein BSP, osteocalcin OCC and receptor activator of nuclear factor kappa-B ligand RANKL.
Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups p=0.049. No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups.
It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.
mandible, mandibular condyle, ethanol, ovariectomy
Granting or Sponsoring Agency
São Paulo State Research Support Foundation
Journal Article Version
Version of Record
Published Article/Book Citation
Braz. J. Oral Sci. vol.14 no.1 Piracicaba Jan./Mar. 2015 DOI: 10.1590/1677-3225v14n1a04
Copyright © 2015
Creative Commons License
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