Document Type

Article

Peer Reviewed

1

Publication Date

3-4-2015

NLM Title Abbreviation

Braz J Oral Sci.

Journal/Book/Conference Title

Brazilian Journal of Oral Sciences

DOI of Published Version

10.1590/1677-3225v14n1a04

Abstract

Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue.

AIM:

To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats.

METHODS:

Fifty-four female rats were first divided equally into two groups: ovariectomized Ovx and simulated ovariectomy Sham. One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein BSP, osteocalcin OCC and receptor activator of nuclear factor kappa-B ligand RANKL.

RESULTS:

Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups p=0.049. No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups.

CONCLUSIONS:

It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.

Keywords

mandible, mandibular condyle, ethanol, ovariectomy

Granting or Sponsoring Agency

São Paulo State Research Support Foundation

Grant Number

2011/03447-0

Journal Article Version

Version of Record

Published Article/Book Citation

Braz. J. Oral Sci. vol.14 no.1 Piracicaba Jan./Mar. 2015 DOI: 10.1590/1677-3225v14n1a04

Rights

Copyright © 2015

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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URL

https://ir.uiowa.edu/dentistry-preventive_pubs/3