Date of Degree
PhD (Doctor of Philosophy)
First Committee Member
Second Committee Member
Third Committee Member
Staphylococcus aureus has been extensively studied, yet it remains unclear why certain individuals continually carry the bacteria while others do not. Livestock workers are known to be at an increased risk of S. aureus colonization, but have not been as studied as other high risk groups, including hospitalized patients, have been. Culture based studies have shown other bacteria may decrease the likelihood of S. aureus colonization. Here, we utilize 16s rRNA sequencing to better characterize the ecologic relationships between S. aureus and the other microbes in the nares and oropharynx in a population of livestock workers.
A cross-sectional, epidemiological study was conducted enrolling 59 participants (26 of which had livestock contact) in Iowa. Participants were enrolled in one of four ways: from an existing prospective cohort study (n=38), from the Iowa Department of Natural Resources Animal Feeding Operations database (n=17), through Iowa county fairs (n=3), and through snowball sampling (n=1). We collected two sets of swabs from the nares and oropharynx of each participant. The first set of swabs was used to assess the microbiome via 16s rRNA sequencing and the second was used to culture S. aureus.
We observed livestock workers to have greater diversity in their microbiomes compared to those with no livestock contact. In the nares, there were 26 operational taxonomic units found to be different between livestock workers and non-livestock workers with the greatest difference seen with Streptococcus and Proteobacteria. In the oropharynx, livestock workers with swine exposure were more likely to carry several pathogenic organisms. We also observed colonized livestock workers to be more likely to carry P. gingivalis which may act as a bridge allowing S. aureus to adhere to Streptococcus in the oral cavity. While we observed no significant differences when comparing colonized persons to non-colonized persons in either the nares or oropharynx, Corynebacterium was more abundant in the colonized persons. Colonized individuals also had greater diversity in their nasal microbiome compared to non-colonized individuals. However, when comparing persistently colonized persons to intermittently colonized persons, we found Corynebacterium argentorantense to be more abundant in the persistently colonized individuals.
We hypothesized the genera present in the nares and oropharynx of S. aureus carriers would be different from that of non-carriers and there would be differences in the nasal and oropharyngeal microbiomes based on livestock contact and carrier state (persistent, intermittent, and non-carrier). While there were no significant differences between carriers and non-carriers, we were able to identify several operational taxonomic units that were different between livestock worker carrier and non-carriers as well as differences by carrier state. The results of this study are the first to characterize the livestock worker nasal and oropharyngeal microbiomes. Additionally, the results shed light onto several organisms that may be influential in S. aureus carriage. However, further studies are needed to better understand these relationships and determine causality.
Staphylococcus aureus is an important cause of infections in the United States and globally and is able to be carried in the nose and throat of healthy people increasing their risk of infection. Livestock workers are at an increased risk of carrying the bacteria. Here we have studied the microbiomes – all of the bacteria present on a body site – of the nose and throat of livestock workers in Iowa. We enrolled 59 participants, 26 of which had contact with livestock. We have identified several bacterial differences in the microbiomes of livestock workers compared to those without livestock contact as well as several bacterial differences in the nose and throat of people colonized with S. aureus compared to those not colonized with S. aureus including Corynebacterium. This study is the first we are aware of to characterize the microbiome of livestock workers which will help us begin to understand if and how the microbiome plays a role in disease. This study also adds to our understanding of what bacteria may impact whether a person carries S. aureus in their nose, throat, or not at all.
16s rRNA, Agriculture medicine, Colonization, Livestock, Mircobiome, Staphylococcus aureus
xiv, 218 pages
Includes bibliographical references (pages 198-218).
Copyright © 2016 Ashley Elizabeth Kates
Kates, Ashley Elizabeth. "The human nasal and oropharyngeal microbiomes and Staphylococcus aureus colonization." PhD (Doctor of Philosophy) thesis, University of Iowa, 2016.