Date of Degree
Access restricted until 07/03/2020
PhD (Doctor of Philosophy)
First Committee Member
Tina L. Tootle
Second Committee Member
Third Committee Member
Fourth Committee Member
Robert E. Malone
Meiosis is a specialized cellular division occurring in organisms capable of sexual reproduction that leads to the formation of gametes containing half of the original chromosome number. Meiosis involves two cell divisions, the first of which segregates homologous chromosomes to opposite poles, reducing ploidy by half. In most organisms, this segregation requires crossovers, the exchange of DNA sequences between homologous chromosomes, which in turn, is dependent upon stable associations of homologs. In early meiotic prophase I, chromosomes form pairing interactions that bring chromosomes into close physical associations. The process of synapsis then stabilizes these pairing interactions throughout the homolog pair, and is mediated by the synaptonemal complex (SC), a meiosis specific protein complex. Absent or misregulated assembly of the SC prevents the stabilization of pairing interactions that are essential for meiosis, leading to chromosome missegregation. Divided into two main projects, my work aimed to further our understanding of the regulation of synaptonemal complex assembly. One project examined meiotic chromosomal movement by characterizing a relatively unstudied protein in C. elegans, FKB-6. We showed that FKB-6 is important for creating pauses between chromosome movements. These pauses are needed for allowing chromosomes to properly pair and thus allowing for proper SC assembly. In the absence of FKB-6, a decrease in pausing occurs which perturbs chromosome pairing and causes SC assembly defects. A second project examined the role of CUL-4, an E3 ubiquitin ligase, in meiotic prophase I. We show that CUL-4 plays a role in both SC assembly and meiotic recombination. This work exemplifies the multiple levels of control of SC assembly which still require further study.
C. elegans, CUL-4, FKB-6, Meiosis, Synaptonemal Complex
xiii, 235 pages
Includes bibliographical references.
Copyright © 2018 Benjamin Alleva
Alleva, Benjamin. "Regulation of synaptonemal complex assembly by the FKB-6 and CUL-4 pathways during meiosis in the model organism Caenorhabditis elegans." PhD (Doctor of Philosophy) thesis, University of Iowa, 2018.
Available for download on Friday, July 03, 2020