Document Type


Date of Degree

Summer 2012

Degree Name

MS (Master of Science)

Degree In

Operative Dentistry

First Advisor

Cobb, Deborah S

Second Advisor

Vargas, Marcos A

First Committee Member

Hernandez, Ma Marcela

Second Committee Member

Kolker, Justine L

Third Committee Member

Qian, Fang


Objective: The aim of this pilot study is the evaluation and measurement of color stability from baseline until stabilization of color associated with bleaching and the dehydration and rehydration process of the teeth.

Method: Six extracted intact human third molars were randomly selected, cleaned, and stored in artificial saliva at 370C. A custom positioning jig was fabricated for each tooth for reproducible color measurement. A baseline shade was recorded according to the Commission Internationale de l'Eclairage L*a*b* (CIE-L*a*b*) using a Minolta Chromameter CR-(200). Color measurement for all testing was taking at baseline, 15 min, 30 min, 2h, 12h and every 2 h thereafter until color stabilization occurred. Phase 1(dehydration-rehydration w/o bleaching): For dehydration, all teeth lightened and color stability occurred at 6 h. Rehydration of teeth to the baseline of color was achieved after 14 hours. Phase 2 (Effect of bleaching): An in-office bleach material (Zoom 25% HP) was applied to each tooth according to the Kwon technique (polyethylene wrap placed to cover the gel) for one hour. All comparisons were made to a Delta value of 2.6 (perceivable difference).

Result: Phase 1: A mean äL*=-13.05(±15.62) was observed for tooth dehydration with no perceivable changes in chroma äa* and äb*. Phase 2: In-office bleaching produced dehydration and chroma changes when measured immediately after bleaching with a mean of äL*=-12.45(±2.4), äa*=0.31(±0.53), and äb*= 3.67(±1.76). After rehydration of bleached teeth, luminosity values (äL*) returned to normal and only chromaticity changes (äb#8727;) were observed with a mean of 4.77(%B11.91).

Conclusion: Within the limits of this study, in-office bleaching produced dehydration and chroma changes, represented by äL* and äb*. Upon rehydration, the color changes were not as discernible. Further study will be conducted to determine the validity of these findings.


Bleaching, Color Changes, Dehydration, In-Office


ix, 98 pages


Includes bibliographical references (pages 86-98).


Copyright 2012 Natalia Restrepo-Kennedy