Document Type


Date of Degree

Spring 2010

Degree Name

MS (Master of Science)

Degree In


First Advisor

Staley, Robert N

First Committee Member

Moreno, Lina

Second Committee Member

Wefel, James

Third Committee Member

Qian, Fang


The purpose of this study was to compare, in vitro, the effectiveness of the resin sealants ProSeal™, OrthoCoat™, SeLECT Defense™, and Biscover LV™ on the inhibition of enamel demineralization after being subjected to extensive mechanical toothbrush abrasion. Seventy-five extracted third molars were divided into five groups - control, ProSeal, SeLECT Defense, OrthoCoat, and Biscover LV.

All of the teeth, with the exception of the control group, weretreated with one of the four sealant materials per the manufacturer's instructions. Teeth were mounted in an acrylic ring and stabilized with a vinyl polysiloxane impression material. The mounted teeth were placed in the Prototech Toothbrush Wear Simulator and a soft-bristled toothbrush was placed over the buccal surface of the tooth.

Each tooth was subjected to 15,000 horizontal strokes at a rate of 120 strokes/minute and each toothbrush received a constant force of 280 g to stimulate normal manual brushing, simulating about 2 years of manual toothbrushing. Teeth from all groups were painted with a thin layer of acid-resistant varnish (nail polish), leaving a 1 mm window of exposed enamel on the buccal surface. They were then placed into a constantly circulating, room-temperature standard tenCate Demineralizing Solution (pH=4.4) consisting of 2.20mM Ca2+, 2.20 mMPO4 3-, 0.05mM Acetic acid and 0.025ppm F- for 96 hours. Three sections were made of each tooth and the sections were examined under a polarized light microscope. Descriptive statistics were conducted with the study data. The one-way ANOVA with post-hoc Ryan-Einot-Gabriel-Welsch multiple range test was used to determine whether there was a significant difference in lesion depth between sealant materials. The following conclusions were made:

All materials tested provided a significant reduction in enamel demineralization as compared to untreated controls. SeLECT Defense and OrthoCoat both resulted in a 64% decrease in lesion depth. Biscover LV provided a 67% decrease in lesion depth. Pro Seal provided significantly more protection (82% reduction) than the other three sealant materials: SeLECT Defense, OrthoCoat, and Biscover LV. Pro Seal, SeLECT Defense, OrthoCoat, and Biscover LV, should all be considered effective preventive treatments that do not require patient compliance for success. Pending further clinical investigation, Pro Seal should still be considered the gold standard for preventing enamel demineralization in orthodontic patients.


vii, 55 pages


Includes bibliographical references (pages 51-55).


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Copyright © 2010 Tanner Jay Clark