Major Department

Health and Human Physiology

College

College of Liberal Arts & Sciences

Degree

BS (Bachelor of Science)

Session and Year of Graduation

Fall 2018

Honors Major Advisor

Dr. Lucas Carr

Thesis Mentor

Dr. Eric J. Devor

Abstract

The microRNA-503 cluster, composed of six microRNAs (miR-424, miR503, miR-542, miR-450a-1, miR450a-2 and miR-450b), is located on the human X-chromosome at Xq26. This location is very near the gene encoding placenta-specific protein 1 (PLAC1) that we have shown is significantly over-expressed in several gynecologic cancers. We examined expression of the miR-503 cluster in a panel of endometrial endometrioid adenocarcinomas (EEAs) and found that all of the miRNAs are significantly under-expressed compared with benign tissue. Expression of the miRNAs is highly correlated suggesting that they are regulated from a single promoter. We have used DNA editing (CRISPR) of the miR-503 cluster promoter to down-regulate the entire cluster together. Additionally, known and validated targets of cluster members include a number of important oncogenes. Thus, suppression of miR-503 cluster members contributes to the establishment and maintenance of endometrial cancer. We further suggest that decreased miR-503 cluster expression is due to hyper-methylation of the promoter in tumor cells. Thus, we conclude that the miR-503 cluster is a potential therapeutic target in endometrial cancer.

Keywords

mir-503, uterine cancer, endometrial endometrioid adenocarcinomas

Total Pages

7 pages

Copyright

Copyright © 2018 Elizabeth Cha

Included in

Oncology Commons

COinS
 

URL

https://ir.uiowa.edu/honors_theses/253