Title

Decreased expression of the c-kit receptor is associated with increased apoptosis in subfertile human testes

Document Type

Article

Peer Reviewed

1

Publication Date

1-1-1999

NLM Title Abbreviation

Fertil Steril

Journal/Book/Conference Title

Fertility and sterility

PubMed ID

9935121

DOI of Published Version

10.1016/S0015-0282(98)00401-4

Start Page

85

End Page

89

Abstract

OBJECTIVE: To examine the expression of the c-kit receptor and its ligand, stem cell factor, and their possible relation with apoptosis in infertile men. DESIGN: Prospective laboratory study. SETTING: Urology laboratory in a university hospital. PATIENT(S): Men undergoing testicular biopsy during an investigation of subfertility. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Expression of the c-kit receptor protein, stem cell factor, and apoptosis in the testes. RESULT(S): The c-kit receptor was strongly present in Leydig cells and type A spermatogonia of normal testes, with decreased staining in Leydig cells and type A spermatogonia of testes with maturational arrest, and staining in only Leydig cells of Sertoli cell-only specimens. Stem cell factor was demonstrated in Leydig cells and Sertoli cells in all specimens. Western blotting demonstrated the 150-kd c-kit protein in the normal testes and the testes with maturational arrest, but not in the testes with the Sertoli cell-only pattern. Stem cell factor was expressed in all specimens, with a protein size of 45 kd. Increased apoptosis was demonstrated in type A spermatogonia and spermatocytes of tissue with maturational arrest compared with normal testicular tissue. CONCLUSION(S): C-kit receptor expression is decreased in subfertile testicular tissue compared with normal testicular tissue. Stem cell factor expression is present in Leydig cells and Sertoli cells. Increased apoptosis is seen in tissue with maturational arrest compared with normal tissue.

Keywords

Adult, Apoptosis/physiology, Blotting, Western, Humans, Immunohistochemistry, In Situ Hybridization, Infertility, Male/metabolism/pathology, Leydig Cells/cytology/metabolism, Ligands, Male, Prospective Studies, Proto-Oncogene Proteins c-kit/biosynthesis, Stem Cell Factor/metabolism, Testis/metabolism/pathology

Published Article/Book Citation

Fertility and sterility, 71:1 (1999) pp.85-89.

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URL

https://ir.uiowa.edu/obgyn_pubs/1067