Title

Identification of a tripartite basal promoter which regulates human terminal deoxynucleotidyl transferase gene expression.

Document Type

Article

Peer Reviewed

1

Publication Date

6-3-1994

Journal/Book/Conference Title

The Journal of biological chemistry

Start Page

15861

End Page

15867

Abstract

In order to locate the promoter region of the human terminal deoxynucleotidyl transferase gene, serially truncated segments of the 5'-flanking region of the gene were cloned into a chloramphenicol acetyltransferase reporter vector. Transient transfection analyses of the terminal transferase-reporter gene constructs identified the basal promoter region within -34 to +40 base pairs relative to the transcription start site. Three promoter elements were defined in this region. The primary element is within 34 base pairs upstream of the transcription start site. The CAP site is 62 base pairs upstream of the translation start site. The secondary element involves sequences around the transcription start site. The third is located 25 base pairs downstream from the initiation site (+25 to +40). This tripartite basal promoter was not tissue specific; similar patterns of promoter activity were observed in terminal transferase expressing and non-expressing cells. Transfection analyses also indicated the presence of negative regulatory elements upstream of the basal promoter region, and these elements were preferentially active in cells expressing terminal transferase.

Keywords

Base Sequence, Cell Line, DNA Nucleotidylexotransferase, DNA Primers, Exons, Gene Expression, Gene Expression Regulation, Enzymologic, Humans, Leukemia, Lymphoma, Molecular Sequence Data, Polymerase Chain Reaction, Promoter Regions, Genetic, RNA, Neoplasm, Transcription, Genetic, Transfection, Tumor Cells, Cultured

Published Article/Book Citation

The Journal of biological chemistry (1994) 269:22, pp. 15861-15867.

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URL

https://ir.uiowa.edu/obgyn_pubs/1176