Document Type

Article

Peer Reviewed

1

Publication Date

6-3-2011

NLM Title Abbreviation

Obstet Gynecol Int

Journal/Book/Conference Title

Obstetrics and gynecology international

DOI of Published Version

10.1155/2011/896896

Start Page

896896

Abstract

We identified a major peptide signaling target of EGF/EGFR pathway and explored the consequences of blocking or activating this pathway in the first trimester extravillous trophoblast cells, HTR-8/SVneo. A global analysis of protein phosphorylation was undertaken using novel technology (Kinexus Kinetworks) that utilizes SDS-polyacrylamide minigel electrophoresis and multi-lane immunoblotting to permit specific and semiquantitative detection of multiple phosphoproteins. Forty-seven protein phosphorylation sites were queried, and the results reported based on relative phosphorylation at each site. EGF- and Iressa-(gefitinib, ZD1839, an inhibitor of EGFR) treated HTR-8/SVneo cells were subjected to immunoblotting and flow cytometry to confirm the phosphoprotein screen and to assess the effects of EGF versus Iressa on cell cycle and apoptosis. EGFR mediates the phosphorylation of important signaling proteins, including PKBα/AKT. This pathway is likely to be central to EGFR-mediated trophoblast survival. Furthermore, EGF treatment induces proliferation and inhibits apoptosis, while Iressa induces apoptosis.

Journal Article Version

Version of Record

Published Article/Book Citation

Obstetrics and gynecology international (2011) 2011:, pp. 896896-896896. https://doi.org/10.1155/2011/896896

Rights

Copyright © 2011 J. Bolnick et al.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

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URL

https://ir.uiowa.edu/obgyn_pubs/51