US Patent 5,300,635
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
The present invention discloses a novel method for isolating nucleic acids from biological samples, most particularly blood, using selected quaternary amine surfactants. The nucleic acids are isolated quickly and in sufficient quantity and quality to permit analysis by methods including reverse transcriptase and polymerase chain reaction.
Macfarlane, Donald E. Quaternary amine surfactants and methods of using same in isolation of nucleic acids. US Patent 5,300,635 filed February 01, 1993 and issued April 05, 1994. https://ir.uiowa.edu/patents/118