Purification of heparinase I, II, and III from Flavobacterium heparinum
US Patent 5,389,539
United States Patent and Trademark Office
Massachusetts Institute of Technology (Cambridge, MA) ; University of Iowa Research Foundation (Iowa City, IA, US)
A purified heparinase I, II and III free of lyase activity and each having a molecular weight of 42,800 84,100, 70,800, respectively, are produced by culturing Flavobacterium heparinum. The kinetic properties of the heparinases have been determined as well as the conditions to optimize their activity and stability.
Sasisekharan, Ramnath; Lohse, Daniel L.; Cooney, Charles L.; Linhardt, Robert J.; and Langer, Robert S. Purification of heparinase I, II, and III from Flavobacterium heparinum. US Patent 5,389,539 filed November 30, 1992 and issued February 14, 1995. https://ir.uiowa.edu/patents/130