Inventor

Douglas H. Jones

Document Type

Patent

Patent Number

US Patent 5,411,875

Patent agency

United States Patent and Trademark Office

Publication Date

5-2-1995

Assignee

University of Iowa Research Foundation (Oakdale, IA, US)

Application Number

07/786,902

Date Filed

1991-11-01

Total Pages

33 pages

Abstract

A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.

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URL

https://ir.uiowa.edu/patents/134