US Patent 5,411,875
United States Patent and Trademark Office
University of Iowa Research Foundation (Oakdale, IA, US)
A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.
Jones, Douglas H. Method for retrieval of unknown flanking DNA sequence. US Patent 5,411,875 filed November 01, 1991 and issued May 02, 1995. https://ir.uiowa.edu/patents/134