US Patent 5,449,613
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
A method for reacting an enzyme in a non-aqueous organic solvent is disclosed. The method comprises preparing a lyophilizate of a salt which activates the enzyme and an enzyme wherein the lyophilizate contains a weight ratio of salt to enzyme of at least 60% salt sufficient to activate the enzyme in an organic solvent. The method then calls for dispersion of the lyophilizate in a non-aqueous organic solvent in the presence of a substrate for the enzyme.
Dordick, Jonathan S.; Khmelnitsky, Yuri; and Clark, Douglas S. Reacting an enzyme in a non-aqueous solvent by adding a lyophilizate of enzyme and salt to the solvent. US Patent 5,449,613 filed March 01, 1994 and issued September 12, 1995. https://ir.uiowa.edu/patents/138