US Patent 5,491,133
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
The use of oligodeoxynucleotides modified at the 3'-terminal internucleotide link as therapeutic agents by a method of hybridizing the modified oligonucleotide to a complementary sequence within a targeted mRNA and cleaving the mRNA within the RNA-DNA helix by the enzyme RNaseH to block the expression of the corresponding gene.
Walder, Joseph A.; Walder, Roxanne Y.; Eder, Paul S.; and Dagle, John M. Methods for blocking the expression of specifically targeted genes. US Patent 5,491,133 filed March 19, 1991 and issued February 13, 1996. https://ir.uiowa.edu/patents/146