Document Type

Patent

Patent Number

US Patent 5,492,839

Patent agency

United States Patent and Trademark Office

Publication Date

2-20-1996

Assignee

University of Iowa Research Foundation (Iowa City, IA, US)

Application Number

08/186,435

Date Filed

1994-01-25

Total Pages

20 pages

Abstract

Disclosed is a novel photo-activatable derivative of ryanodine 9-hydroxy-21-(4-azidobenzoyloxy)-9-epiryanodine. This novel ryanodine derivative has been conjugated to keyhole limpet hemocyanin (KLH) and used for the production of antibodies with high affinity and specificity to ryanodine. A radioimmunoassay specific for ryanodine was developed using the anti-ryanodine antibodies, and a dissociation constant for ryanodine of 1 nM was determined. Also disclosed is the binding of a labeled, photoactivatable derivative of ryanodine (ABRy) with the ryanodine receptor of skeletal, cardiac and brain membranes. Digestion of the labeled ryanodine receptor revealed a labeled 76 kDa tryptic fragment which is critical for the formation of the high affinity ryanodine binding site.

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URL

https://ir.uiowa.edu/patents/148