US Patent 5,569,600
United States Patent and Trademark Office
Massachusetts Institute of Technology (Cambridge, MA) ; University of Iowa Research Foundation (Iowa City, IA, US)
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
Sasisekharan, Ramnath; Lohse, Daniel L.; Cooney, Charles L.; Linhardt, Robert J.; and Langer, Robert S. Purification, composition and specificity of heparinase I, II, and III from flavobacterium heparinum. US Patent 5,569,600 filed January 26, 1995 and issued October 29, 1996. https://ir.uiowa.edu/patents/154