US Patent 5,759,835
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
Nitric oxide synthase has been discovered to exist in bacteria. The bacterial nitric oxide synthase was purified as much as 1,362-fold by a combination of 2', 5'-ADP-agarose affinity chromatography, and hydroxylapatite chromatography and its unique N-terminal amino acid sequence was identified.
Rosazza, John P. and Chen, Yijun Bacterial nitric oxide synthase. US Patent 5,759,835 filed September 12, 1995 and issued June 02, 1998. https://ir.uiowa.edu/patents/183