US Patent 5,856,158
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
A nitric oxide synthase (NOS) protein is obtained from rat brain and purified to a specific activity at least 6,360-fold that of a crude tissue preparation. The NOS protein has a denatured MW of 105 kD determined by SDS-PAGE, a dimeric MW of 230 kD determined by gel filtration and requires FAD, FMN, Ca2+ and tetrahydropterin for the production of nitric oxide from L-arginine or an arginine-rich peptide, oligopeptide or protein substrate.
Rosazza, John P. N. and Chen, Yijun Purified nitric oxide synthase from rat brain. US Patent 5,856,158 filed July 05, 1996 and issued January 05, 1999. https://ir.uiowa.edu/patents/201