US Patent 6,171,813
United States Patent and Trademark Office
BioTechnology Research & Develop. Corp. (Peoria, IL) ; University of Iowa Research Foundation (Iowa City, IA, US)
A method is presented for catalyzing the conversion of substrate into product in an organic reaction solvent with an enzyme-surfactant ion pair. The enzyme-surfactant ion pair comprises the enzyme catalyzing the reaction and a surfactant capable of forming an ion pair with the enzyme. Water present in the organic solvent at a concentration of about 0.03% to about 2.5% is sufficient to enhance the rate of catalysis and stabilize the enzyme without substantially increasing the rate of hydrolysis when compared to the anhydrous enzyme.
Dordick, Jonathan S.; Paradkar, Vikram M.; and Sergeeva, Maria V. Enzyme catalysis in organic solutions containing water. US Patent 6,171,813 filed October 16, 1997 and issued January 09, 2001. https://ir.uiowa.edu/patents/253