US Patent 6,190,889
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.
Jones, Douglas H. Methods for removing primer sequences and blocking restriction endonuclease recognition domains. US Patent 6,190,889 filed January 07, 1999 and issued February 20, 2001. https://ir.uiowa.edu/patents/257