US Patent 6,316,228
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
Methods and compositions, including cell cultures, for producing nucleosides are provided which comprise contacting a nucleoside precursor and a sugar moiety donor with a cell containing a nucleoside phosphorylase. The nucleoside precursor can be a purine or pyrimidine base and the sugar moiety donor can comprise a ribose, a deoxyribose, including 2-deoxyribose, or other sugar of choice. The methods and composition can be used to make thymidine from thymine and 2-deoxyinosine or 2'-deoxyadenosine. Other nucleosides, including those having anticancer and/or antiviral properties, also can be obtained according to the invention.
Nair, Vasu and Pal, Suresh Efficient synthesis of nucleosides. US Patent 6,316,228 filed July 12, 1999 and issued November 13, 2001. https://ir.uiowa.edu/patents/291