Document Type

Patent

Patent Number

US Patent 4,403,036

Patent agency

United States Patent and Trademark Office

Publication Date

9-6-1983

Assignee

University of Iowa Research Foundation (Iowa City, IA, US)

Application Number

06/212,254

Date Filed

1980-12-02

Total Pages

10 pages

Abstract

Genetic reagents for generating plasmids containing multiples of a DNA segment are prepared by modification of the restriction sites of plasmid rings. The modified plasmids permit cloning of DNA segments which can be recovered with sticky ends that are complementary but not rotationally equivalent. Such segments will polymerize in a head-to-tail conformation. The plasmid rings with modified restriction sites are also used in linear form to obtain head-to-tail joining of multiple DNA segments into plasmid rings for further cloning and/or expression of the DNA segment-directed protein. The critical restriction site sequences of the modified plasmid rings can be prepared as a reagent which permits the sequence to be introduced into any plasmid. The reagents have utility in preparing multiples of protein-forming genes, and in preparing large amounts of homogeneous DNAs which can themselves be used as reagents.

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URL

https://ir.uiowa.edu/patents/33