US Patent 6,821,957
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
The present invention shows that DNA vaccine vectors can be improved by removal of CpG-N motifs and optional addition of CpG-S motifs. In addition, for high and long-lasting levels of expression, the optimized vector should include a promoter/enhancer that is not down-regulated by the cytokines induced by the immunostimulatory CpG motifs. Vectors and methods of use for immununostimulation are provided herein. The invention also provides improved gene therapy vectors by determining the CpG-N and CpG-S motifs present in the construct, removing stimulatory CpG (CpG-S) motifs and/or inserting neutralizing CpG (CpG-N) motifs, thereby producing a nucleic acid construct providing enhanced expression of the therapeutic polypeptide. Methods of use for such vectors are also included herein.
Krieg, Arthur M.; Davis, Heather L.; Wu, Tong; and Joachim, Schorr Vectors and methods for immunization or therapeutic protocols. US Patent 6,821,957 filed September 26, 2001 and issued November 23, 2004. https://ir.uiowa.edu/patents/365