US Patent 6,887,483
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US) ; The Regents of the University of California (Oakland, CA, US)
A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional HtrB protein, resulting in a mutant that lacks one or more secondary acyl chains and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, or the endotoxin isolated therefrom, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.
Apicella, Michael A.; Sunshine, Melvin G.; Lee, Na-gyong; and Gibson, Bradford W. Non-toxic mutants of pathogenic gram-negative bacteria. US Patent 6,887,483 filed December 01, 1995 and issued May 03, 2005. https://ir.uiowa.edu/patents/375