US Patent 7,005,129
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US) ; The Regents of the University of California (Oakland, CA, US)
A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional htrB protein, resulting in a mutant that lacks one or more secondary acyl chains contained in the wild type gram-negative bacterial pathogen, and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, the endotoxin isolated therefrom, or the endotoxin isolated therefrom which is then conjugated to a carrier protein, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.
Apicella, Michael A.; Sunshine, Melvin G.; Lee, Na-gyong; Gibson, Bradford W.; and Arumugham, Rasappa Non-toxic mutants of pathogenic gram-negative bacteria. US Patent 7,005,129 filed November 27, 1996 and issued February 28, 2006. https://ir.uiowa.edu/patents/391