Document Type


Patent Number

US Patent 7,460,895

Patent agency

United States Patent and Trademark Office

Publication Date



University of Iowa Research Foundation (Iowa City, IA, US)

Application Number


Date Filed


Total Pages

19 pages


A method for generating a net analyte signal calibration model for use in detecting and/or quantifying the amount of an analyte in a test subject. The net analyte signal can be generated by providing a set of in vivo infrared spectra for a test subject during a period in which an analyte concentration is essentially constant; calculating an optimal subspace of spectra that at least substantially describes all non-analyte dependent spectral variance in the in vivo spectra; providing a pure component infrared spectrum for the analyte; and calculating a net analyte signal spectrum from a data set comprising the optimal subspace spectra and the pure analyte spectrum. The net analyte signal calibration model can be used, for example, in measuring the concentration of analyte in a test subject, and/or for evaluating the analytical significance of an in vivo multivariate calibration model.

Creative Commons License

Creative Commons License
This work has been identified with a Creative Commons Public Domain Mark 1.0.