US Patent 7,795,380
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
Compositions and methods are described for non-viral nucleic acid delivery. A targeting peptide capable of mediating targeting to a cell or subcellular compartment is derivatized with a photoaffinity label. Following an ionic interaction with a polynucleotide, such as DNA, and photolysis, the bioactive peptide becomes covalently attached to the DNA. Upon contact with a cell, the peptide facilities uptake of the peptide-polynucleotide conjugate into the cell or subcellular compartment. Methods for using this system for delivery of structural genes, including reporter genes, and detection of expression using bioluminescence are also described.
Rice, Kevin G.; Rettig, Garrett R.; and Baumhover, Nicholas J. Compositions and methods for nucleic acid delivery. US Patent 7,795,380 filed June 04, 2007 and issued September 14, 2010. https://ir.uiowa.edu/patents/521