Document Type

Patent

Patent Number

US Patent 7,795,380

Patent agency

United States Patent and Trademark Office

Publication Date

9-14-2010

Assignee

University of Iowa Research Foundation (Iowa City, IA, US)

Application Number

11/757,848

Date Filed

2007-06-04

Total Pages

48 pages

Abstract

Compositions and methods are described for non-viral nucleic acid delivery. A targeting peptide capable of mediating targeting to a cell or subcellular compartment is derivatized with a photoaffinity label. Following an ionic interaction with a polynucleotide, such as DNA, and photolysis, the bioactive peptide becomes covalently attached to the DNA. Upon contact with a cell, the peptide facilities uptake of the peptide-polynucleotide conjugate into the cell or subcellular compartment. Methods for using this system for delivery of structural genes, including reporter genes, and detection of expression using bioluminescence are also described.

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URL

https://ir.uiowa.edu/patents/521