US Patent 7,795,380
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
Compositions and methods are described for non-viral nucleic acid delivery. A targeting peptide capable of mediating targeting to a cell or subcellular compartment is derivatized with a photoaffinity label. Following an ionic interaction with a polynucleotide, such as DNA, and photolysis, the bioactive peptide becomes covalently attached to the DNA. Upon contact with a cell, the peptide facilities uptake of the peptide-polynucleotide conjugate into the cell or subcellular compartment. Methods for using this system for delivery of structural genes, including reporter genes, and detection of expression using bioluminescence are also described.
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Rice, Kevin G; Rettig, Garrett R; and Baumhover, Nicholas J Compositions and methods for nucleic acid delivery. US Patent 7,795,380 filed June 04, 2007 and issued September 14, 2010. https://ir.uiowa.edu/patents/521