US Patent 8,232,257
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US)
Embodiments of the present invention provide methods for targeted inactivation of viral genomes. In one embodiment, zinc-finger proteins in which DNA binding sites are altered such that they recognize and bind different, desired DNA sequences contained in hepatitis B virus (HBV) and that include nuclease domains are used for inactivation. Other embodiments for targeted inactivation of viral genomes use small nucleic acid molecules, such as short micro-RNA molecules or short hairpin RNA molecules capable of mediating RNA interference (RNAi) against the hepatitis B virus.
Mccaffrey, Anton P. and Cradick, Thomas J. RNA interference mediated inactivation of hepatitis B virus in a subject. US Patent 8,232,257 filed March 27, 2008 and issued July 31, 2012. https://ir.uiowa.edu/patents/577