US Patent 8,703,469
United States Patent and Trademark Office
Boyce Thompson Institute for Plant Research (Ithaca, NY, US) ; University of Iowa Research Foundation (Iowa City, IA, US)
A method of co-expressing a portion of the VSV G protein gene or a truncated “stem” portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.
Mccray, Paul; Sinn, Patrick; and Blissard, Gary Baculoviruses with enhanced virion production and a method for the production of baculoviruses. US Patent 8,703,469 filed September 13, 2011 and issued April 22, 2014. https://ir.uiowa.edu/patents/619