Document Type

Patent

Patent Number

US Patent 8,703,469

Patent agency

United States Patent and Trademark Office

Publication Date

4-22-2014

Assignee

Boyce Thompson Institute for Plant Research (Ithaca, NY, US) ; University of Iowa Research Foundation (Iowa City, IA, US)

Application Number

13/231,324

Date Filed

2011-09-13

Total Pages

27 pages

Abstract

A method of co-expressing a portion of the VSV G protein gene or a truncated “stem” portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.

Share

COinS
 

URL

https://ir.uiowa.edu/patents/619