Document Type

Patent

Patent Number

US Patent 9,603,949

Patent agency

United States Patent and Trademark Office

Publication Date

3-28-2017

Assignee

University of Iowa Research Foundation (Iowa City, IA, US) ; Integrated DNA Technologies, Inc. (Coralville, IA, US)

Application Number

14/342,338

Date Filed

2012-08-30

Total Pages

49 pages

Abstract

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.

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URL

https://ir.uiowa.edu/patents/684