US Patent 9,603,949
United States Patent and Trademark Office
University of Iowa Research Foundation (Iowa City, IA, US) ; Integrated DNA Technologies, Inc. (Coralville, IA, US)
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.
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McNamara, James O II; Stockdale, Katie R; Huang, Lingyan; Horswill, Alexander R; Behlke, Mark A; and Hernandez, Frank J Oligonucleotide-based probes for detection of bacterial nucleases. US Patent 9,603,949 filed August 30, 2012 and issued March 28, 2017. https://ir.uiowa.edu/patents/684