Document Type

Patent

Patent Number

US Patent 4,933,281

Patent agency

United States Patent and Trademark Office

Publication Date

6-12-1990

Assignee

University of Iowa Research Foundation (Iowa City, IA, US)

Application Number

07/026,857

Date Filed

1987-03-17

Total Pages

8 pages

Abstract

A method for large-scale production of rhamnose and 3-hydroxydecanoic acid is disclosed comprising the steps of growing microorganisms of Pseudomonas sp. capable of production of high levels of rhamnolipid in a defined culture medium containing vegetable oil. Additional steps include isolating the rhamnolipid from the culture medium, hydrolyzing the rhamnolipid to produce rhamnose and 3-hydroxydecanoic acid, and separating the rhamnose from the acid. Corn oil is the preferred vegetable oil and Pseudomonas aeruginosa is the preferred Pseudomonas sp. Non-limiting concentrations of nitrogen compounds and magnesium compounds and limiting concentrations of iron compounds are additionally preferably included in the culture medium.

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URL

https://ir.uiowa.edu/patents/76