Document Type

Article

Peer Reviewed

1

Publication Date

3-2018

NLM Title Abbreviation

Am J Ophthalmol

Journal/Book/Conference Title

American journal of ophthalmology

PubMed ID

29294312

DOI of Published Version

10.1016/j.ajo.2017.12.017

Start Page

51

End Page

60

Abstract

PURPOSE: To examine whether herpes zoster antigen (also called varicella-zoster virus antigen) was detectable in temporal artery biopsies taken from individuals with giant cell arteritis (GCA).

DESIGN: Retrospective comparative case series.

METHODS: Sections of formalin-fixed paraffin-embedded temporal arteries were examined first by hematoxylin-eosin (H&E) staining to establish the diagnosis of GCA. Adjacent sections of the same biopsy were then examined by immunohistochemistry, using 2 different monoclonal antibodies against a major antigen of varicella-zoster virus called gE. Pathologic specimens were obtained from patients cared for at the University of Iowa and Washington University in St. Louis ophthalmology clinics.

RESULTS: The study included biopsies from 25 patients with symptoms of GCA as well as positive H&E pathology and 25 patients with symptoms compatible with GCA but negative H&E pathology. Among the GCA-positive group, 3 patients had positive staining for herpes zoster antigen. Among the GCA-negative group, herpes zoster antigen was not detected in any biopsy. In both groups of patients, false-positive staining for herpes zoster antigen was detected in the presence of calcifications in the arteries. False-positive staining was also detected on some extra-arterial skeletal muscle and erythrocytes.

CONCLUSION: Herpes zoster antigen was detected in 3 of 25 temporal arteries from patients with biopsy-proven GCA. One of the 3 positive cases was noteworthy because the patient had had herpes zoster ophthalmicus diagnosed 3 weeks before the onset of GCA symptoms. False-positive staining for herpes zoster antigen was detected on several temporal artery biopsies.

Keywords

Herpes Zoster, Giant cell arteritis, Pediatrics, Immunohistochemistry, Ophthalmology, Virology

Comments

RESEARCH ON GCA AT THE UNIVERSITY OF IOWA WAS SUPPORTED BY GRANTS FROM THE IOWA CITY VA

Center for the Prevention and Treatment of Visual Loss C-9251-C RR&D and the National Shingles Foundation, New York, New York. Varicella reagents were produced during research supported by NIH grant AI89716, Bethesda, Maryland. Research at Washington University in St. Louis was supported in part by an NIH Core Grant (EY002687) and an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology and Visual Sciences

Journal Article Version

Version of Record

Published Article/Book Citation

Am J Ophthalmol. 2018 Mar;187:51-60. doi: https://doi.org/10.1016/j.ajo.2017.12.017

Rights

© 2017 The Authors

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Share

COinS
 

URL

https://ir.uiowa.edu/pediatrics_pubs/15