Document Type

Article

Peer Reviewed

1

Publication Date

11-7-2018

NLM Title Abbreviation

Genes (Basel)

Journal/Book/Conference Title

Genes

PubMed ID

30405068

DOI of Published Version

10.3390/genes9110538

Start Page

538

Total Pages

23

Abstract

Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a cAMP-regulated anion channel. Although CF is a multi-organ system disease, most people with CF die of progressive lung disease that begins early in childhood and is characterized by chronic bacterial infection and inflammation. Nearly 90% of people with CF have at least one copy of the ΔF508 mutation, but there are hundreds of CFTR mutations that result in a range of disease severities. A CFTR gene replacement approach would be efficacious regardless of the disease-causing mutation. After the discovery of the CFTR gene in 1989, the in vitro proof-of-concept for gene therapy for CF was quickly established in 1990. In 1993, the first of many gene therapy clinical trials attempted to rescue the CF defect in airway epithelia. Despite the initial enthusiasm, there is still no FDA-approved gene therapy for CF. Here we discuss the history of CF gene therapy, from the discovery of the CFTR gene to current state-of-the-art gene delivery vector designs. While implementation of CF gene therapy has proven more challenging than initially envisioned; thanks to continued innovation, it may yet become a reality.

Keywords

adeno-associated virus, pediatrics, adenovirus, animal models, clinical trials, history, lentivirus, non-viral vectors, retrovirus, viral vectors

Comments

This work was supported by the National Institutes of Health [NIH P01 HL-51670, NIH P01 HL-091842, NIH R01 HL-133089, NIH R01 HL-105821]; the Center for Gene Therapy of Cystic Fibrosis [NIH P30 DK-054759]; and the Cystic Fibrosis Foundation [SINN15XX0]. Funding for open access charge: National Institutes of Health. Additional support by the Roy J. Carver Endowed Chair in Lung Research.

Journal Article Version

Version of Record

Published Article/Book Citation

Genes (Basel). 2018 Nov 7;9(11). pii: E538. doi: https://doi.org/10.3390/genes9110538

Rights

© 2018 by the authors.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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URL

https://ir.uiowa.edu/pediatrics_pubs/21