Objective: To assess the effect of gonadotropin-releasing hormone analogue (GnRHa) on the preservation of ovarian function against cyclophosphamide-induced gonadal toxicity.
Materials and Methods: In a controlled, experimental study, 64 female mice were divided into four groups: control (C), triptorelin acetate (T), cyclophosphamide (CY), and triptorelin plus cyclophosphamide (T+CY) groups. Mice in the group (T) were subcutaneously injected with GnRHa (triptorelin acetate) in a dose of 0.5 mg/kg daily for 21 days. In contrast, mice in the (CY) group and (T+CY) group were injected intraperitoneally with 75 mg/kg of CY on day 15. After 21 days, half of the mice in each group were sacrificed, and their ovaries were removed. The rest of the mice in each group were left without any intervention for an additional 21 days, and the same procedures were repeated to assess the ovarian follicles.
Results:There was signiﬁcant depletion of ovarian follicles in the CY group compared to the control group (p
Conclusion: Cyclophosphamide destroys primordial and primary follicles at an early stage while damage in secondary and antral follicles was prominent after 42 days. Triptorelin acetate reduces the toxic effect of CY; it has early and late protective effects and preserves ovarian function in mice.
GnRH analogue, cyclophosphamide, ovarian toxicity, ovarian function
The authors report no conflict of interest.
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Department of Obstetrics and Gynecology, The University of Iowa Hospitals and Clinics
Kishk EA, Hassan WA, Shaaban MM, Abbas AM. The effect of gonadotropin-releasing hormone analogues on the preservation of ovarian function against cyclophosphamide-induced damage in adult mice. POG in Press. 2019 September; Article 1 [14 p.]. Available from: http://ir.uiowa.edu/pog_in_press/. Free full text article.